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Budding yeasts were observed using an FE-SEM equipped with a cryo system. The yeasts were dropped directly onto a sample stub, rapidly frozen with slush nitrogen without prior fixation or dehydration, then cleaved and coated with 2 nm of Pt in the preparation chamber of the cryo system. Invagination structure unique to yeast cell membranes were observed in the exposed protoplasmic fracture (PF) face. In the enlarged view, particles with a diameter of approximately 15 nm were recognized and some
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Budding yeasts were observed using an FE-SEM equipped with a …
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And it's a dead heat! They're checking the electron microscope. And the winner is... number 3, in a quantum finish. 'No fair! You changed the outcome by measuring it.' (S3 E4 - 'The Luck of the Fryrish') | The Simpsons & Futurama Best Moments
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