Mouse tissue samples (∼50 mg) underwent homogenization in 200 uL of ice-cold RIPA buffer (consisting of 150 mM NaCl ... mouse anti-FLAG-M2-tag antibody (Sigma–Aldrich, F3165) diluted 1:2000 in 5% milk ...
Nuclear cell staining was done with Hoechst 33342 (Sigma-Aldrich). Cell preparations were analyzed in ... Sample Preparation, SDS-PAGE, and Western Immunoblotting Cell lysates were prepared in a RIPA ...
After dehydration, specimens were embedded in epoxy resin (Epon 812), (Sigma-Aldrich Co., St. Louis ... the cells in 100 μl of radioimmunoprecipitation assay lysis buffer (RIPA buffer), (Santa Cruz ...
Loss function of tumor suppressor FRMD8 confers resistance to tamoxifen therapy via a dual mechanism
Anti-Flag (#F3165) antibody was from Sigma-Aldrich. Antibodies against GAPDH (#AC002 ... Cells or tissues were lysed using RIPA buffer (1×PBS, pH 7.4, 0.5% sodium deoxycholate, 1% NP-40, and 0.1% SDS) ...
Kelly Ripa and Mark Consuelos shared their son's story on their TV show Ripa and Consuelos shared their story while chatting to Rita Ora, who's a native of London, specifically of the famous ...
Kelly Ripa and Mark Consuelos are apparently keeping it traditional in their home. On Live with Kelly and Mark on Wednesday, Ripa revealed that they line their vegetable crisper with a row of ...
Watch: Kelly Ripa Is Unrecognizable as Ariana Grande in 'Wicked' Transformation And while the 54-year-old disappeared into Ariana’s Oscar-nominated role, it’s certainly not the first time she ...
Kelly Ripa has been hailed the queen of cosplay as she transformed into Ariana Grande with epic proportions on Tuesday. The LIVE with Kelly and Mark co-anchor underwent a major makeover as she ...
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