Modular cloning and expression of complex genetic assemblies are becoming widely popular. Recent applications based on site-specific and homologous recombination have substantially improved the ...

Homing endonucleases are usually encoded within self-splicing introns or inteins, and are found in representatives of all three kingdoms 1,2. The endonuclease genes and their host intron or intein are ...

Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...

Once pure samples of DNA have been prepared, it can be manipulated in several ways; cut at specific sites and then joined together in a controlled manner. They also can be shortened, lengthened, ...