CRISPR genome editing generates double-stranded breaks (DSBs) in genomic DNA and is a targeted method by which to achieve gene knockouts and knock-ins. Endogenous repair of DSBs results in an array of ...

Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...

Scientists from the United States have recently identified a novel approach to rapidly and accurately detect the omicron BA.2.12.1 sub-lineage of severe acute respiratory syndrome coronavirus 2 ...

Limited sample material and insufficient DNA input pose significant challenges for downstream analysis in various laboratory settings. To overcome this issue, isothermal amplification techniques have ...

A new form of PCR has been used to improve the library preparation for forensic DNA typing from difficult samples like touch evidence and human remains. Typically, DNA sequencing and analysis is ...

Microzone in the United Kingdom has recently introduced microLYSIS, a reagent designed to lyse a wide variety of different cell types prior to PCR. MicroLYSIS is a proprietary mixture of detergents ...

Today, PCR is ubiquitous in molecular biology laboratories. As one of the first experiments that young scientists master, it is easy to take for granted. But developing this research lab staple ...

Fast, flexible and easy-to-use qScript Ultra Flex Kit and qScript Ultra SuperMix enable scientists to reverse transcribe longer targets and maximize yields from challenging samples in only 10 minutes ...

Researchers at the Chan Zuckerberg Biohub, San Francisco, and collaborators have warned against only targeting one viral gene when carrying out reverse transcriptase-polymerase chain reaction (RT-PCR) ...