Design a CRISPR construct to knockout TAT genes in cyanobacteria. Identify the optimal CRISPR plasmid backbone, design and insert the guide RNA (gRNA) into the plasmid, synthesize and insert repair ...
CRISPR-Cas9 has been widely adapted for use in transcriptional modulation and epigenetic engineering with deactivated Cas9 (dCas9) systems to enable CRISPR interference (CRISPRi) and CRISPR activation ...
Investigators at CHOP and UPenn found that lower cell counts could still be associated with poor outcomes if the cells were poorly differentiated.
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